Coding

Part:BBa_K1137001:Design

Designed by: Matthew Deyell   Group: iGEM13_Paris_Bettencourt   (2013-09-23)


M. Smegmatis FprA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 948
    Illegal AgeI site found at 310
    Illegal AgeI site found at 928
    Illegal AgeI site found at 1252
    Illegal AgeI site found at 1306
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

High homology to FprA from M. Tuberculosis Codon Optimized for E. coli

Source

Synthesized from M. Smegmatis genome.

References

McLEAN. K.J., SCRUTTON. N.S. and MUNRO, A.W. (2005) Kinetic, spectroscopic and thermodynamic characterization of the Mycobacterium tuberculosis adrenodoxin reductase homologue FprA Biochem. J. (2003) 372 (317–327) (Printed in Great Britain) doi:10.1042/BJ20021692