Coding
Part:BBa_K1137001:Design
Designed by: Matthew Deyell Group: iGEM13_Paris_Bettencourt (2013-09-23)
M. Smegmatis FprA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 948
Illegal AgeI site found at 310
Illegal AgeI site found at 928
Illegal AgeI site found at 1252
Illegal AgeI site found at 1306 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
High homology to FprA from M. Tuberculosis Codon Optimized for E. coli
Source
Synthesized from M. Smegmatis genome.
References
McLEAN. K.J., SCRUTTON. N.S. and MUNRO, A.W. (2005) Kinetic, spectroscopic and thermodynamic characterization of the Mycobacterium tuberculosis adrenodoxin reductase homologue FprA Biochem. J. (2003) 372 (317–327) (Printed in Great Britain) doi:10.1042/BJ20021692